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Defining the immune mechanism with monoclonal antibodies

Academic Article
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Overview

authors

  • Klinman, Norman R.
  • Denis, K. A.
  • Sherman, Linda

publication date

  • 1981

journal

  • In Vitro-Journal of the Tissue Culture Association  Journal

abstract

  • It has long been realized that only the study of homogeneous antibodies or cell populations could enable a definitive understanding of much of the immune mechanism. Hybridoma technology has greatly facilitated such approaches. Hybridoma antibodies have been used to delineate both B cell and T cell subpopulations. T cell studies per se have been accomplished by the use of T cell hybridoma cell lines producing a variety of factors. Anti-idiotypes against B cell hybridoma antibodies have been used to characterize T cell receptors and factors. B cell studies have been facilitated by hybridomas that have made available the immunoglobulin of pre-B cells or defective B cell lines. Hybridoma antibodies have also been used to dissect closely related antibody families and the potential for responsiveness against a variety of antigenic determinants. Finally, hybridomas have provided a primary source of material for protein and DNA sequence analysis. In our laboratories hybridoma antibodies derived against the murine H-2 locus have demonstrated the ability of B cell antibodies to discriminate amongst H2 mutants--a capacity previously attributed only to T cell specificities. Hybridoma antibodies have also been generated by fusions with antigen stimulated neonatal B cells to provide homogeneous antibodies reflective of the earliest developmental immunoglobulin readout. Such probes should increase our understanding of the processes involved in the generation of both the T and B cell repertoires

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antibody Formation
  • Antigens, Surface
  • B-Lymphocytes
  • Epitopes
  • H-2 Antigens
  • Hemagglutinins, Viral
  • Hybridomas
  • Immunoglobulin Idiotypes
  • Immunoglobulins
  • Macrophages
  • T-Lymphocytes
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Identity

International Standard Serial Number (ISSN)

  • 0073-5655

PubMed ID

  • 6172363
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Additional Document Info

start page

  • 1029

end page

  • 1035

volume

  • 17

issue

  • 12

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