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Synthesis of plasma membrane-associated and secretory immunoglobulin in diploid lymphocytes

Academic Article
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Overview

authors

  • Lerner, Richard
  • McConahey, P. J.
  • Jansen, I.
  • Dixon, F. J.

publication date

  • 1972

journal

  • Journal of Experimental Medicine  Journal

abstract

  • The half disappearance time for detectable plasma membrane-associated and cytoplasmic immunoglobulin after treatment of continuously growing diploid lymphocytes with inhibitors of protein and RNA synthesis was studied. Also, the amount of plasma membrane-associated and cytoplasmic immunoglobulin of synchronized cells in the G(1) phase of the cell cycle has been studied. Plasma membrane-associated immunoglobulin has a half disappearance time of 45 min after inhibition of protein synthesis. By contrast, after treatment of cells with actinomycin D for 24 hr, plasma membrane-associated immunoglobulin remains relatively unchanged whereas cytoplasmic immunoglobulin decreased by almost 90%. In the G(1) phase of the cell cycle, plasma membrane-associated immunoglobulin and cytoplasmic immunoglobulin were 70 and 10%, respectively, of that in logarithmically growing cells, and the half disappearance of M-Ig after treatment of cells with puromycin was again 45 min. In toto, these results suggest that perhaps secreted and plasma membrane-associated immunoglobulin may be separately controlled by the cells.

subject areas

  • Animals
  • Cell Membrane
  • Cytoplasm
  • Diploidy
  • Humans
  • Immunoglobulins
  • Lymphocytes
  • Rabbits
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Identity

PubMed Central ID

  • PMC2139120

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.135.1.136

PubMed ID

  • 5061901
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Additional Document Info

start page

  • 136

end page

  • 149

volume

  • 135

issue

  • 1

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