Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Selection of monoclonal antibodies for linear epitopes of an apolipoprotein yields antibodies with comparable affinity for lipid-free and lipid-associated apolipoprotein

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Curtiss, Linda
  • Banka, C. L.

publication date

  • April 1996

journal

  • Journal of Lipid Research  Journal

abstract

  • A direct approach is described for generating human apolipoprotein (apo) A-I-specific monoclonal antibodies with defined linear epitopes. The antibodies have comparable binding affinity for delipidated apoA-I and apoA-I on high density lipoproteins (HDL). The antibodies were obtained by immunizing mice with HDL, screening the fusions first for antibodies that bind native HDL and second, for antibodies that bind defined apoA-I synthetic peptides. ApoA-I antibody epitopes assigned on the basis of synthetic peptide binding were confirmed by solid phase and fluid phase antibody competition assays. These antibodies, which bind epitopes that encompass greater than 60% of apoA-I, should prove useful for identifying discrete functional domains of apoA-I on HDL.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antibody Affinity
  • Apolipoprotein A-I
  • Apolipoproteins
  • Binding, Competitive
  • Epitopes
  • Humans
  • Hybridomas
  • Lipids
  • Lipoproteins, HDL
  • Mice
  • Molecular Structure
scroll to property group menus

Research

keywords

  • apolipoprotein A-I
  • liner epitopes
  • monoclonal antibodies
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-2275

PubMed ID

  • 8732788
scroll to property group menus

Additional Document Info

start page

  • 884

end page

  • 892

volume

  • 37

issue

  • 4

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support