Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Recognition of the mrna au-rich element by the zinc finger domain of tis11d

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Hudson, B. P.
  • Martinez-Yamout, M. A.
  • Dyson, Jane
  • Wright, Peter

publication date

  • March 2004

journal

  • Nature Structural & Molecular Biology  Journal

abstract

  • The tandem zinc finger (TZF) domain of the protein TIS11d binds to the class II AU-rich element (ARE) in the 3' untranslated region (3' UTR) of target mRNAs and promotes their deadenylation and degradation. The NMR structure of the TIS11d TZF domain bound to the RNA sequence 5'-UUAUUUAUU-3' comprises a pair of novel CCCH fingers of type CX(8)CX(5)CX(3)H separated by an 18-residue linker. The two TIS11d zinc fingers bind in a symmetrical fashion to adjacent 5'-UAUU-3' subsites on the single-stranded RNA via a combination of electrostatic and hydrogen-bonding interactions, with intercalative stacking between conserved aromatic side chains and the RNA bases. Sequence specificity in RNA recognition is achieved by a network of intermolecular hydrogen bonds, mostly between TIS11d main-chain functional groups and the Watson-Crick edges of the bases. The TIS11d structure provides insights into the RNA-binding functions of this large family of CCCH zinc finger proteins.

subject areas

  • 3' Untranslated Regions
  • Adenine
  • Base Composition
  • Binding Sites
  • DNA-Binding Proteins
  • Humans
  • Hydrogen Bonding
  • Immediate-Early Proteins
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Binding
  • RNA, Messenger
  • Static Electricity
  • Tristetraprolin
  • Uracil
  • Zinc Fingers
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1545-9985

Digital Object Identifier (DOI)

  • 10.1038/nsmb738

PubMed ID

  • 14981510
scroll to property group menus

Additional Document Info

start page

  • 257

end page

  • 264

volume

  • 11

issue

  • 3

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support