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Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin

Academic Article
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Overview

authors

  • Beutler, Bruce
  • Milsark, I. W.
  • Cerami, A. C.

publication date

  • 1985

journal

  • Science  Journal

abstract

  • A highly specific polyclonal rabbit antiserum directed against murine cachectin/tumor necrosis factor (TNF) was prepared. When BALB/c mice were passively immunized with the antiserum or with purified immune globulin, they were protected against the lethal effect of the endotoxin lipopolysaccharide produced by Escherichia coli. The prophylactic effect was dose-dependent and was most effective when the antiserum was administered prior to the injection of the endotoxin. Antiserum to cachectin/TNF did not mitigate the febrile response of endotoxin-treated animals, and very high doses of endotoxin could overcome the protective effect. The median lethal dose of endotoxin in mice pretreated with 50 microliters of the specific antiserum was approximately 2.5 times greater the median lethal dose for controls given nonimmune serum. The data suggest that cachectin/TNF is one of the principal mediators of the lethal effect of endotoxin.

subject areas

  • Animals
  • Endotoxins
  • Escherichia coli
  • Female
  • Glycoproteins
  • Immune Sera
  • Immunization, Passive
  • Lethal Dose 50
  • Lipopolysaccharides
  • Mice
  • Mice, Inbred BALB C
  • Proteins
  • Tumor Necrosis Factor-alpha
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.3895437

PubMed ID

  • 3895437
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Additional Document Info

start page

  • 869

end page

  • 871

volume

  • 229

issue

  • 4716

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