Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Persistent hepatitis C virus infection in vitro: Coevolution of virus and host

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Zhong, J.
  • Gastaminza, P.
  • Chung, J.
  • Stamataki, Z.
  • Isogawa, Masanori
  • Cheng, G. F.
  • McKeating, J. A.
  • Chisari, Francis

publication date

  • November 2006

journal

  • Journal of Virology  Journal

abstract

  • The virological and cellular consequences of persistent hepatitis C virus (HCV) infection have been elusive due to the absence of the requisite experimental systems. Here, we report the establishment and the characteristics of persistent in vitro infection of human hepatoma-derived cells by a recently described HCV genotype 2a infectious molecular clone. Persistent in vitro infection was characterized by the selection of viral variants that displayed accelerated expansion kinetics, higher peak titers, and increased buoyant densities. Sequencing analysis revealed the selection of a single adaptive mutation in the HCV E2 envelope protein that was largely responsible for the variant phenotype. In parallel, as the virus became more aggressive, cells that were resistant to infection emerged, displaying escape mechanisms operative at the level of viral entry, HCV RNA replication, or both. Collectively, these results reveal the existence of coevolutionary events during persistent HCV infection that favor survival of both virus and host.

subject areas

  • Adaptation, Biological
  • Cell Line, Tumor
  • DNA Mutational Analysis
  • Hepacivirus
  • Hepatocytes
  • Humans
  • Mutation, Missense
  • RNA, Viral
  • Sequence Analysis, DNA
  • Viral Envelope Proteins
  • Virus Internalization
  • Virus Replication
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.01307-06

PubMed ID

  • 16956932
scroll to property group menus

Additional Document Info

start page

  • 11082

end page

  • 11093

volume

  • 80

issue

  • 22

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support