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Applications of dapi cytochemistry to neurobiology

Academic Article
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Overview

authors

  • Sanna, Pietro
  • Jirikowski, G. F.
  • Lewandowski, G. A.
  • Bloom, Floyd

publication date

  • November 1992

journal

  • Biotechnic & Histochemistry  Journal

abstract

  • 4',6-Diamidino-2-phenylindole hydrochloride (DAPI) is a fluorescent dye with high affinity for DNA. We have employed it as a fluorescent chromatin counterstain on sections immunofluorescent-stained using rhodamine and on tissues enzymatically stained using beta-galactosidase. DAPI also allows easy identification of mitotic figures and can be used to supplement cytochemical studies involving cell division in the nervous system.

subject areas

  • Animals
  • Cell Division
  • Cell Nucleus
  • Central Nervous System
  • Chromatin
  • DNA
  • Fluorescent Antibody Technique
  • Fluorescent Dyes
  • Histocytochemistry
  • Indoles
  • Mice
  • Mitosis
  • Neurobiology
  • Rats
  • beta-Galactosidase
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Research

keywords

  • CYTOCHEMISTRY
  • DAPI
  • IMMUNOFLUORESCENCE
  • NERVOUS SYSTEM
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Identity

International Standard Serial Number (ISSN)

  • 1052-0295

Digital Object Identifier (DOI)

  • 10.3109/10520299209110047

PubMed ID

  • 1489836
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Additional Document Info

start page

  • 346

end page

  • 350

volume

  • 67

issue

  • 6

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