Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Regulation of receptor-mediated endocytosis by rho and rac

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Lamaze, C.
  • Chuang, T. H.
  • Terlecky, L. J.
  • Bokoch, G. M.
  • Schmid, Sandra

publication date

  • July 1996

journal

  • Nature  Journal

abstract

  • Pinocytosis and membrane ruffling are among the earliest and most dramatic cellular responses to stimulation by growth factors or other mitogens. The small Ras-related G proteins Rho and Rac have a regulatory role in membrane ruffling and activated Rho has been shown to stimulate pinocytosis when microinjected into Xenopus oocytes. In contrast to these well established effects of Rho and Rac on plasma membrane morphology and bulk pinocytosis, there has been no evidence for their involvement in the regulation of receptor-mediated endocytosis in clathrin-coated pits. Here we show that activated Rho and Rac inhibit transferrin-receptor-mediated endocytosis when expressed in intact cells. Furthermore, we have reconstituted these effects in a cell-free system and established that Rho and Rac can regulate clathrin-coated vesicle formation.

subject areas

  • Cell Line
  • Clathrin
  • Coated Vesicles
  • Cytochalasin D
  • Endocytosis
  • GTP-Binding Proteins
  • HeLa Cells
  • Humans
  • Mutation
  • Phalloidine
  • Receptors, Transferrin
  • Recombinant Proteins
  • Transferrin
  • rac GTP-Binding Proteins
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/382177a0

PubMed ID

  • 8700210
scroll to property group menus

Additional Document Info

start page

  • 177

end page

  • 179

volume

  • 382

issue

  • 6587

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support