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The phage-t4 uvsy recombination protein stabilizes presynaptic filaments

Academic Article
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Overview

authors

  • Kodadek, Thomas
  • Gan, D. C.
  • Stemkehale, K.

publication date

  • October 1989

journal

  • Journal of Biological Chemistry  Journal

abstract

  • The bacteriophage T4 uvsY protein is required for efficient recombination in T4-infected Escherichia coli cells. Previous in vitro work has shown that the purified uvsY protein is an accessory protein; it stimulates homologous pairing catalyzed by the phage uvsX protein (a RecA-like recombinase) under certain conditions. We show here that this effect can be traced, at least in part, to a UvsY-dependent stabilization of uvsX protein-single-stranded DNA complexes. These presynaptic filaments are one of the early obligatory intermediates in the strand exchange reaction between homologous single- and double-stranded DNAs. The mechanism of filament stabilization seems to involve a slower loss of UvsX subunits. A model that accounts for the data is presented in which both recombination proteins are incorporated into the presynaptic filament.

subject areas

  • Adenosine Triphosphatases
  • Chromatography
  • Chromatography, Ion Exchange
  • DNA-Binding Proteins
  • Durapatite
  • Escherichia coli
  • Hydroxyapatites
  • Kinetics
  • Membrane Proteins
  • Models, Theoretical
  • Recombination, Genetic
  • T-Phages
  • Viral Proteins
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 2550444
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Additional Document Info

start page

  • 16451

end page

  • 16457

volume

  • 264

issue

  • 28

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