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Comparison of the native ccmv virion with in vitro assembled ccmw virions by cryoelectron microscopy and image reconstruction

Academic Article
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Overview

authors

  • Fox, J. M.
  • Wang, G. J.
  • Speir, J. A.
  • Olson, N. H.
  • Johnson Jr., John
  • Baker, T. S.
  • Young, M. J.

publication date

  • 1998

journal

  • Virology  Journal

abstract

  • Cryoelectron microscopy and three-dimensional image reconstruction analysis has been used to determine the structure of native and in vitro assembled cowpea chlorotic mottle virus (CCMV) virions and capsids to 25-A resolution. Purified CCMV coat protein was used in conjunction with in vitro transcribed viral RNAs to assemble RNA 1 only, RNA 2 only, RNA 3/4 only, and empty (RNA lacking) virions. The image reconstructions demonstrate that the in vitro assembled CCMV virions are morphologically indistinguishable from native virions purified from infected plants. The viral RNA (vRNA) is packaged similarly within the different types of virions. The centers of all assembled particles are generally devoid of density and the vRNA packs against the interior surface of the virion shell. The vRNA appears to adopt an ordered conformation at each of the quasi-threefold axes.

subject areas

  • Bromovirus
  • Capsid
  • Image Processing, Computer-Assisted
  • Microscopy, Electron
  • Plants
  • RNA, Viral
  • Virion
  • Virus Assembly
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Research

keywords

  • CCMV
  • cryoelectron microscopy
  • virus assembly
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Identity

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1006/viro.1998.9107

PubMed ID

  • 9581792
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Additional Document Info

start page

  • 212

end page

  • 218

volume

  • 244

issue

  • 1

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