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Pheromone-induced phosphorylation of a g-protein beta-subunit in saccharomyces-cerevisiae is associated with an adaptive response to mating pheromone

Academic Article
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Overview

authors

  • Cole, G. M.
  • Reed, Steven

publication date

  • February 1991

journal

  • Cell  Journal

abstract

  • The mating pheromone response in S. cerevisiae is activated by a G protein-mediated signaling pathway in which G beta gamma is the active transducer of the signal. When exogenous pheromone is added to vegetatively growing cells, G beta is rapidly phosphorylated at several sites; phosphorylation does not require de novo protein synthesis. A mutation in G beta was constructed that eliminates signal-induced phosphorylation. This mutation leads to enhanced sensitivity to and impaired ability to recover from pheromone, but does not affect the ability of G beta gamma to transmit the mating signal. These phenotypes suggest that G protein phosphorylation mediates an adaptive response to pheromone-induced signaling. G beta phosphorylation does not require either the pheromone receptor C-terminus or the product of the SST2 gene, both of which mediate separate adaptive responses to pheromone. However, G beta phosphorylation is greatly facilitated by the presence of the G alpha subunit, which has also been shown to participate in an adaptation to pheromone.

subject areas

  • Amino Acid Sequence
  • Amino Acids
  • Chromosome Deletion
  • Crosses, Genetic
  • GTP-Binding Protein beta Subunits
  • GTP-Binding Proteins
  • Genes, Fungal
  • Genotype
  • Heterotrimeric GTP-Binding Proteins
  • Macromolecular Substances
  • Molecular Sequence Data
  • Peptides
  • Pheromones
  • Phosphoproteins
  • Phosphorylation
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Nucleic Acid
  • Signal Transduction
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(91)90500-x

PubMed ID

  • 1900039
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Additional Document Info

start page

  • 703

end page

  • 716

volume

  • 64

issue

  • 4

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