CSI-FID: high throughput label-free detection of DNA binding molecules

Academic Article

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Overview

authors

• Hauschild, K. E.
• Stover, J. S.
• Boger, Dale
• Ansari, A. Z.

publication date

• July 2009

journal

• Bioorganic & Medicinal Chemistry Letters  Journal

abstract

• Determining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the sequence specificity of DNA ligands using label free detection on high density DNA microarrays. This is achieved by combining Cognate Site Identification (CSI) with Fluorescence Intercalation Displacement (FID) to create CSI-FID. We use the well-studied small molecule DNA ligand netropsin to develop this high throughput platform. Analysis of the DNA binding properties of protein- and small molecule-based libraries with CSI-FID will advance the development of genome-anchored molecules for therapeutic purposes.

subject areas

• Binding Sites
• DNA
• Ethidium
• Fluorescent Dyes
• Intercalating Agents
• Ligands
• Netropsin
• Oligonucleotide Array Sequence Analysis
• Small Molecule Libraries

Research

keywords

• CSI microarray
• FID
• Label-free detection
• Small-molecule specificity

Identity

PubMed Central ID

• PMC2760306

International Standard Serial Number (ISSN)

• 0960-894X

Digital Object Identifier (DOI)

• 10.1016/j.bmcl.2009.04.097

PubMed ID

• 19435662

Additional Document Info

start page

• 3779

end page

• 3782

volume

• 19

issue

• 14