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Defining the Pseudomonas aeruginosa SOS response and its role in the global response to the antibiotic ciprofloxacin

Academic Article
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Overview

related to degree

  • Cirz, Ryan T, Ph.D. in Biology, Scripps Research 2002 - 2007
  • O'Neill, Bryan M, Ph.D. in Biology, Scripps Research 2001 - 2007

authors

  • Cirz, Ryan T
  • O'Neill, Bryan M
  • Hammond, J. A.
  • Head, Steve
  • Romesberg, Floyd

publication date

  • October 2006

journal

  • Journal of Bacteriology  Journal

abstract

  • Pseudomonas aeruginosa infections can be virtually impossible to eradicate, and the evolution of resistance during antibiotic therapy is a significant concern. In this study, we use DNA microarrays to characterize the global transcriptional response of P. aeruginosa to clinical-like doses of the antibiotic ciprofloxacin and also to determine the component that is regulated by LexA cleavage and the SOS response. We find that genes involved in virtually every facet of metabolism are down-regulated in response to ciprofloxacin. The LexA-controlled SOS regulon identified by microarray analysis includes only 15 genes but does include several genes that encode proteins involved in recombination and replication, including two inducible polymerases known to play a role in mutation and the evolution of antibiotic resistance in other organisms. The data suggest that the inhibition of LexA cleavage during therapy might help combat this pathogen by decreasing its ability to adapt and evolve resistance.

subject areas

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Ciprofloxacin
  • DNA-Directed DNA Polymerase
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Oligonucleotide Array Sequence Analysis
  • Pseudomonas aeruginosa
  • RNA, Bacterial
  • RNA, Messenger
  • Recombinases
  • Regulon
  • SOS Response (Genetics)
  • Serine Endopeptidases
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Identity

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.00807-06

PubMed ID

  • 17015649
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Additional Document Info

start page

  • 7101

end page

  • 7110

volume

  • 188

issue

  • 20

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