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Pseudomonas aeruginosa outer-membrane protein f epitopes are highly immunogenic in mice when expressed an a plant virus

Academic Article
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Overview

authors

  • Brennan, F. R.
  • Jones, T. D.
  • Gilleland, L. B.
  • Bellaby, T.
  • Xu, F.
  • North, P. C.
  • Thompson, A.
  • Staczek, J.
  • Lin, T.
  • Johnson Jr., John
  • Hamilton, W. D. O.
  • Gilleland, H. E.

publication date

  • January 1999

journal

  • Microbiology-SGM  Journal

abstract

  • A synthetic peptide (peptide 10) representing a surface-exposed, linear B cell epitope from outer-membrane (OM) protein F of Pseudomonas aeruginosa was shown previously to afford protection in mice from P. aeruginosa infection. This peptide was expressed in tandem with the protein F peptide 18 on each of the two coat proteins of cowpea mosaic virus (CPMV). The chimaeric virus particles (CVPs) expressing the peptides on the S (small) coat protein (CPMV-PAE4) and L (large) coat protein (CPMV-PAE5) were used to immunize mice. Following subcutaneous immunization in Freund's and QuilA adjuvants, CPMV-PAE4 induced antibodies predominantly against peptide 18, whereas CPMV-PAE5 produced antibodies exclusively against peptide 10, indicating that the site of peptide expression on CPMV influences its immune recognition. The anti-peptide antibodies elicited by CPMV-PAE5 were predominantly of the IgG2a isotype, indicating a highly polarized TH1-type response. The peptide-specific IgG2a strongly recognized the whole F protein, but more importantly, recognized protein F in all seven Fisher-Devlin immunotypes of P. aeruginosa. Furthermore, the peptide-specific IgG2a in CVP/QS-21 adjuvant-immunized mice was shown to bind complement and to augment phagocytosis of P. aeruginosa by human neutrophils in vitro. The ability of CPMV-PAE5 to induce P. aeruginosa-specific opsonic IgG2a gives it potential for further development as a protective vaccine against P. aeruginosa.

subject areas

  • Animals
  • Antibodies, Bacterial
  • Capsid
  • Comovirus
  • Complement System Proteins
  • Epitopes
  • Genetic Vectors
  • Humans
  • Immunization
  • Immunoglobulin G
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Neutrophils
  • Opsonin Proteins
  • Phagocytosis
  • Porins
  • Pseudomonas Infections
  • Pseudomonas aeruginosa
  • Th1 Cells
  • Vaccines, Synthetic
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Research

keywords

  • Pseudomonas aeruginosa
  • chimaeric virus particle
  • cowpea mosaic virus
  • outer-membrane protein F
  • vaccine
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Identity

International Standard Serial Number (ISSN)

  • 1350-0872

PubMed ID

  • 10206701
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Additional Document Info

start page

  • 211

end page

  • 220

volume

  • 145

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