Single-crystals of cadmium, zinc metallothionein

Academic Article

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Overview

authors

• Melis, K. A.
• Carter, D. C.
• Stout, C. David
• Winge, D. R.

publication date

• 1983

journal

• Journal of Biological Chemistry  Journal

abstract

• Single crystals have been grown of Cd,Zn metallothionein isoform II from rat liver. The space group is P4(1)2(1)2 (P4(3)2(1)2) with unit cell dimensions a = b = 31.0 A and c = 120.0 A, and one molecule in the crystallographic asymmetric unit. The crystals are square bipyramids elongated on the tetragonal c-axis and are grown by repetitive seeding. The crystals are suitable for high resolution structure analysis. Assays of dissolved crystals show that the crystals have the same Cd and Zn content and amino acid composition as the native, as-isolated protein.

subject areas

• Animals
• Cadmium
• Crystallization
• Liver
• Metalloproteins
• Metallothionein
• Rats
• X-Ray Diffraction
• Zinc

Identity

International Standard Serial Number (ISSN)

• 0021-9258

PubMed ID

• 6853483

Additional Document Info

start page

• 6255

end page

• 6257

volume

• 258

issue

• 10