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Recovery of soluble sheep erythrocyte receptor from the T lymphocyte surface by proteolytic cleavage

Academic Article
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Overview

authors

  • Chisari, Francis
  • Gealy, W. J.
  • Edgington, Thomas

publication date

  • 1977

journal

  • Journal of Immunology  Journal

abstract

  • Proteolytic digestion of the human T lymphoblastoid cell line (Molt-4) and of peripheral blood lymphocytes by trypsin, chymotrypsin, and pronase results in a progressive, time-and dose-dependent diminution of T lymphocyte-sheep red bloock cell (SRBC) rosette formation, whereas thrombin, plasmin, collagenase, DNAse, and phospholipase have not effect. Complete abrogation of SRBC binding is achieved when lymphocytes (1 x 108/ml) are incubated with either trypsin or chymotrypsin at 10 mug/ml for 30 min, and greater than 50% abrogation is observed between 3 to 10 min. Preincubation of SRBC with the 10 min and 20 min lymphocyte digest supernatants inhibited their subsequent binding by normal T lymphocytes by as much as 64%. Thirty-minute digests were less inhibitory. Equivalent digests from several human B lumphoblastoid cell lines and from a non-rosetting clone of Molt-4 cells were not inhibitory. Polyacrylamide gel electrophoresis followed by elution of serial gel slices revealed four distinct inhibitory bands (I-IV) in the 20-min digest supernatant whereas only bands I-III and band IV were present in the 10-min and 30-min digest supernatants, respectively, suggesting progressive proteolysis of a distinct receptor. These experiments indicate that the binding of SRBC by human T lymphocytes represents a receptor-ligand interaction rather than a nonspecific electrical charge phe nomenon and that the receptor is a discrete molecular species which can be isolated from the surface of T but not B lymphocytes by limited enzymatic proteolysis.

subject areas

  • Binding Sites
  • Cell Line
  • Cell Membrane
  • Chymotrypsin
  • Erythrocytes
  • In Vitro Techniques
  • Membrane Proteins
  • Peptide Hydrolases
  • Pronase
  • Protein Binding
  • T-Lymphocytes
  • Trypsin
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 300398
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Additional Document Info

start page

  • 1138

end page

  • 1142

volume

  • 118

issue

  • 4

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