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Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes

Academic Article
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Overview

authors

  • Padgett, R. A.
  • Konarska, M. M.
  • Aebi, M.
  • Hornig, H.
  • Weissmann, Charles
  • Sharp, P. A.

publication date

  • 1985

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Mutants of the rabbit beta-globin gene lacking the natural site of branch formation in the second intervening sequence have been analyzed for in vitro splicing activity. RNAs transcribed from these mutants were spliced, via lariat formation, at a reduced rate compared to wild-type RNA. The sites of branch formation were mapped by direct RNA analysis and primer-extension analysis. The sequences at the branch sites in the three mutants examined did not conform to the previously determined consensus sequence, nor were the 5' splice sites and branch sites complementary.

subject areas

  • Animals
  • Base Sequence
  • DNA, Recombinant
  • Genes
  • Globins
  • Kinetics
  • Mutation
  • RNA Splicing
  • Rabbits
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Identity

PubMed Central ID

  • PMC390913

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.82.24.8349

PubMed ID

  • 3866228
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Additional Document Info

start page

  • 8349

end page

  • 8353

volume

  • 82

issue

  • 24

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