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Future prospects for the analysis of complex biological systems using micro-column liquid chromatography-electrospray tandem mass spectrometry

Academic Article
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Overview

authors

  • Yates III, John
  • McCormack, A. L.
  • Link, A. J.
  • Schieltz, D.
  • Eng, J.
  • Hays, L.

publication date

  • July 1996

journal

  • Analyst  Journal

abstract

  • An overview is provided of methods for the study of complex biological processes by using micro-column liquid chromatography-electrospray ionization tandem mass spectrometry. Procedures discussed include electrospray ionization, micro-column liquid chromatography, tandem mass spectrometry, tandem mass spectra data interpretation for peptides, and database searching with mass spectral data. Several problems in immunology are discussed to illustrate this approach.

subject areas

  • Amino Acid Sequence
  • Animals
  • Chromatography, Liquid
  • Humans
  • Mass Spectrometry
  • Molecular Biology
  • Molecular Sequence Data
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Research

keywords

  • database
  • electrospray
  • peptide
  • protein
  • review
  • tandem mass spectrometry
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Identity

International Standard Serial Number (ISSN)

  • 0003-2654

Digital Object Identifier (DOI)

  • 10.1039/an996210065r

PubMed ID

  • 8757920
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Additional Document Info

start page

  • 65R

end page

  • 76R

volume

  • 121

issue

  • 7

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