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Kinetic and stability properties of Penicillium chrysogenum ATP sulfurylase missing the C-terminal regulatory domain

Academic Article
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Overview

authors

  • Hanna, E.
  • Ng, K. F.
  • MacRae, Ian
  • Bley, C. J.
  • Fisher, A. J.
  • Segel, I. H.

publication date

  • February 2004

journal

  • Journal of Biological Chemistry  Journal

abstract

  • ATP sulfurylase from Penicillium chrysogenum is a homohexameric enzyme that is subject to allosteric inhibition by 3'-phosphoadenosine 5'-phosphosulfate. In contrast to the wild type enzyme, recombinant ATP sulfurylase lacking the C-terminal allosteric domain was monomeric and noncooperative. All kcat values were decreased (the adenosine 5'-phosphosulfate (adenylylsulfate) (APS) synthesis reaction to 17% of the wild type value). Additionally, the Michaelis constants for MgATP and sulfate (or molybdate), the dissociation constant of E.APS, and the monovalent oxyanion dissociation constants of dead end E.MgATP.oxyanion complexes were all increased. APS release (the k6 step) was rate-limiting in the wild type enzyme. Without the C-terminal domain, the composite k5 step (isomerization of the central complex and MgPPi release) became rate-limiting. The cumulative results indicate that besides (a) serving as a receptor for the allosteric inhibitor, the C-terminal domain (b) stabilizes the hexameric structure and indirectly, individual subunits. Additionally, (c) the domain interacts with and perfects the catalytic site such that one or more steps following the formation of the binary E.MgATP and E.SO4(2-) complexes and preceding the release of MgPPi are optimized. The more negative entropy of activation of the truncated enzyme for APS synthesis is consistent with a role of the C-terminal domain in promoting the effective orientation of MgATP and sulfate at the active site.

subject areas

  • Allosteric Site
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal
  • Enzyme Inhibitors
  • Enzyme Stability
  • Hydrogen-Ion Concentration
  • Kinetics
  • Models, Molecular
  • Molecular Weight
  • Penicillium chrysogenum
  • Phosphoadenosine Phosphosulfate
  • Protein Structure, Tertiary
  • Protein Subunits
  • Recombinant Proteins
  • Sequence Deletion
  • Sulfate Adenylyltransferase
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M311317200

PubMed ID

  • 14613928
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Additional Document Info

start page

  • 4415

end page

  • 4424

volume

  • 279

issue

  • 6

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