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Performance of a linear ion trap-Orbitrap hybrid for peptide analysis

Academic Article
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Overview

authors

  • Yates III, John
  • Cociorva, D.
  • Liao, L. J.
  • Zabrouskov, V.

publication date

  • January 2006

journal

  • Analytical Chemistry  Journal

abstract

  • Proteomic analysis of digested complex protein mixtures has become a useful strategy to identify proteins involved in biological processes. We have evaluated the use of a new mass spectrometer that combines a linear ion trap and an Orbitrap to create a hybrid tandem mass spectrometer. A digested submandibular/sublingual saliva sample was used for the analysis. We find the instrument is capable of mass resolution in excess of 40,000 and mass measurement accuracies of less than 2 ppm for the analysis of complex peptide mixtures. Such high mass accuracy allowed the elimination of virtually any false positive peptide identifications, suggesting that peptides that do not match the specificity of the protease used in the digestion of the sample should not automatically be considered as false positives. Tandem mass spectra from the linear ion trap and from the Orbitrap have very similar ion abundance ratios. We conclude this instrument will be well suited for shotgun proteomic types of analyses.

subject areas

  • Humans
  • Metalloendopeptidases
  • Proteomics
  • Saliva
  • Salivary Proteins and Peptides
  • Tandem Mass Spectrometry
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Identity

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac0514624

PubMed ID

  • 16408932
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Additional Document Info

start page

  • 493

end page

  • 500

volume

  • 78

issue

  • 2

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