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Progress toward an expanded eukaryotic genetic code

Academic Article
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Overview

authors

  • Chin, J. W.
  • Cropp, T. A.
  • Chu, S.
  • Meggers, E.
  • Schultz, Peter

publication date

  • June 2003

journal

  • Chemistry & Biology  Journal

abstract

  • Expanding the eukaryotic genetic code to include unnatural amino acids with novel properties would provide powerful tools for manipulating protein function in eukaryotic cells. Toward this goal, a general approach with potential for isolating aminoacyl-tRNA synthetases that incorporate unnatural amino acids with high fidelity into proteins in Saccharomyces cerevisiae is described. The method is based on activation of GAL4-responsive HIS3, URA3, or lacZ reporter genes by suppression of amber codons in GAL4. The optimization of GAL4 reporters is described, and the positive and negative selection of active Escherichia coli tyrosyl-tRNA synthetase (EcTyrRS)/tRNA(CUA) is demonstrated. Importantly, both selections can be performed on a single cell and with a range of stringencies. This method will facilitate the isolation of a range of aminoacyl-tRNA synthetase (aaRS)/tRNA(CUA) activities from large libraries of mutant synthetases.

subject areas

  • Amino Acids
  • Amino Acyl-tRNA Synthetases
  • Escherichia coli
  • Eukaryotic Cells
  • Genes, Reporter
  • Genetic Code
  • Genetic Techniques
  • Protein Biosynthesis
  • Proteins
  • RNA, Transfer, Amino Acyl
  • Saccharomyces cerevisiae
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 1074-5521

Digital Object Identifier (DOI)

  • 10.1016/s1074-5521(03)00123-6

PubMed ID

  • 12837384
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Additional Document Info

start page

  • 511

end page

  • 519

volume

  • 10

issue

  • 6

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