Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Characterization of the spoivb and recn loci of bacillus-subtilis

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Vanhoy, B. E.
  • Hoch, James

publication date

  • 1990

journal

  • Journal of Bacteriology  Journal

abstract

  • Two independent genes, recN and spoIVB, along with their respective promoter and termination regions, were discovered and sequenced in the 3.4-kilobase region between the ahrC and spoOA genes at map position 216 in the Bacillus subtilis chromosome map. The gene encoding a 576-amino-acid protein, which maintains a high homology with the Escherichia coli recN gene product, was adjacent to ahrC. The sequence revealed a 64,472-dalton polypeptide which contained a conserved ATP-binding site and possible lexA-type regulatory binding sequences in its promoter region. A second open reading frame identified as the spoIVB gene was directly downstream of recN. It consisted of 1,275 nucleotides which coded for a 425-amino-acid polypeptide with a molecular weight of 45,976. Phenotypic, genetic, and transcriptional analyses confirmed that this gene was spoIVB. Although no chloroform-resistant spores were produced by spoIVB-inactivated strains, under microscopic examination, phase-gray forespores were visible. The spoIVB165 mutation was localized to a 200-base-pair region in the amino-terminal portion of the polypeptide, spoIVB was not transcribed until hour 2 of sporulation in wild-type B. subtilis cells, as determined by beta-galactosidase activity assays from lacZ transcriptional fusion constructions. We found no amino acid sequence homology between the spoIVB gene product and other known bacterial proteins.

subject areas

  • Amino Acid Sequence
  • Bacillus subtilis
  • Bacterial Proteins
  • Base Sequence
  • DNA Restriction Enzymes
  • Escherichia coli
  • Genes, Bacterial
  • Genes, Regulator
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Spores, Bacterial
  • Terminator Regions, Genetic
  • Transcription Factors
  • Transcription, Genetic
scroll to property group menus

Identity

PubMed Central ID

  • PMC208599

International Standard Serial Number (ISSN)

  • 0021-9193

PubMed ID

  • 2106508
scroll to property group menus

Additional Document Info

start page

  • 1306

end page

  • 1311

volume

  • 172

issue

  • 3

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support