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Binding of monoclonal-antibody to the epstein-barr virus (ebv)/cr2 receptor induces activation and differentiation of human lymphocytes-b

Academic Article
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Overview

authors

  • Nemerow, Glen
  • McNaughton, M. E.
  • Cooper, N. R.

publication date

  • 1985

journal

  • Journal of Immunology  Journal

abstract

  • A panel of B cell-specific monoclonal antibodies that identify the CR2/EBV receptor were examined for their ability to mimic the T-independent mitogenic agent, EBV, and thus activate human peripheral blood B lymphocytes. Two of four different anti-CR2/EBV monoclonal antibodies, OKB7 and AB-1, produced a 50-fold to 200-fold dose-dependent stimulation of DNA synthesis of peripheral blood mononuclear cells. One of the other monoclonal antibodies, anti-B2, had slight activity, and the other, HB-5, was completely inactive. One of the mitogenic antibodies, OKB7, which directly inhibits binding and infection of B cells by EBV in the absence of a second anti-immunoglobulin antibody, was examined in further detail. Both the intact antibody in soluble form and its pepsin-derived F(ab')2 fragment stimulated DNA synthesis of unseparated B and T lymphocytes. Peak stimulation of DNA synthesis in peripheral blood mononuclear cells occurred between 4 to 6 days. B cells were responsible for incorporation of [3H]thymidine. However, T cells were required for activation of peripheral blood mononuclear cells by OKB7. OKB7, as well as the other mitogenic monoclonal anti-EBV/CR2 receptor antibody, also induced B cells to differentiate after 6 to 10 days of culture as indicated by polyclonal Ig secretion. IgM was the predominate immunoglobulin secreted. These studies thus indicate that certain epitopes on the EBV/CR2 receptor trigger B cells to divide and differentiate. This pathway of B cell activation, in contrast to that produced by EBV, is T cell dependent.

subject areas

  • Adult
  • Antibodies, Monoclonal
  • B-Lymphocytes
  • Binding Sites, Antibody
  • Cell Differentiation
  • Complement C3
  • DNA
  • Herpesvirus 4, Human
  • Humans
  • Lymphocyte Activation
  • Monocytes
  • Receptors, Complement
  • Receptors, Complement 3d
  • Receptors, Virus
  • T-Lymphocytes
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 2995485
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Additional Document Info

start page

  • 3068

end page

  • 3073

volume

  • 135

issue

  • 5

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