Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Shotgun proteomics in neuroscience

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Liao, L. J.
  • McClatchy, D. B.
  • Yates III, John

publication date

  • July 2009

journal

  • Neuron  Journal

abstract

  • Mass spectrometry-based proteomics is increasingly used to address basic and clinical questions in biomedical research through studies of differential protein expression, protein-protein interactions, and posttranslational modifications. The complex structural and functional organization of the human brain warrants the application of high-throughput, systematic approaches to understand the functional alterations under normal physiological conditions and the perturbations of neurological diseases. This primer focuses on shotgun-proteomics-based tandem mass spectrometry for the identification of proteins in a complex mixture. It describes the basic concepts of protein differential expression analysis and posttranslational modification analysis and discusses several strategies to improve the coverage of the proteome.

subject areas

  • Animals
  • Brain
  • Databases, Protein
  • Humans
  • Mass Spectrometry
  • Neurosciences
  • Protein Interaction Mapping
  • Proteome
  • Proteomics
scroll to property group menus

Identity

PubMed Central ID

  • PMC2746002

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2009.06.011

PubMed ID

  • 19607789
scroll to property group menus

Additional Document Info

start page

  • 12

end page

  • 26

volume

  • 63

issue

  • 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support