Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Functional properties of a new voltage-dependent calcium channel alpha(2)delta auxiliary subunit gene (cacna2d2)

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Gao, B. N.
  • Sekido, Y.
  • Maximov, Anton
  • Saad, M.
  • Forgacs, E.
  • Latif, F.
  • Wei, M. H.
  • Lerman, M.
  • Lee, Jiing-Dwan
  • Perez-Reyes, E.
  • Bezprozvanny, I.
  • Minna, J. D.

publication date

  • 2000

journal

  • Journal of Biological Chemistry  Journal

abstract

  • We have positionally cloned and characterized a new calcium channel auxiliary subunit, alpha(2)delta-2 (CACNA2D2), which shares 56% amino acid identity with the known alpha(2)delta-1 subunit. The gene maps to the critical human tumor suppressor gene region in chromosome 3p21.3, showing very frequent allele loss and occasional homozygous deletions in lung, breast, and other cancers. The tissue distribution of alpha(2)delta-2 expression is different from alpha(2)delta-1, and alpha(2)delta-2 mRNA is most abundantly expressed in lung and testis and well expressed in brain, heart, and pancreas. In contrast, alpha(2)delta-1 is expressed predominantly in brain, heart, and skeletal muscle. When co-expressed (via cRNA injections) with alpha(1B) and beta(3) subunits in Xenopus oocytes, alpha(2)delta-2 increased peak size of the N-type Ca(2+) currents 9-fold, and when co-expressed with alpha(1C) or alpha(1G) subunits in Xenopus oocytes increased peak size of L-type channels 2-fold and T-type channels 1.8-fold, respectively. Anti-peptide antibodies detect the expression of a 129-kDa alpha(2)delta-2 polypeptide in some but not all lung tumor cells. We conclude that the alpha(2)delta-2 gene encodes a functional auxiliary subunit of voltage-gated Ca(2+) channels. Because of its chromosomal location and expression patterns, CACNA2D2 needs to be explored as a potential tumor suppressor gene linking Ca(2+) signaling and lung, breast, and other cancer pathogenesis. The homologous location on mouse chromosome 9 is also the site of the mouse neurologic mutant ducky (du), and thus, CACNA2D2 is also a candidate gene for this inherited idiopathic generalized epilepsy syndrome.

subject areas

  • Amino Acid Sequence
  • Animals
  • Calcium Channels
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Mice
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Xenopus laevis
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.275.16.12237

PubMed ID

  • 10766861
scroll to property group menus

Additional Document Info

start page

  • 12237

end page

  • 12242

volume

  • 275

issue

  • 16

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support