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Identification of ntf2, a cytosolic factor for nuclear import that interacts with nuclear-pore complex protein p62

Academic Article
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Overview

authors

  • Paschal, B. M.
  • Gerace, Larry

publication date

  • May 1995

journal

  • Journal of Cell Biology  Journal

abstract

  • Protein import into the nucleus is a multistep process that requires the activities of several cytosolic factors. In this study we have purified a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62. Isolation involved biochemical complementation of cytosol depleted of this activity by preadsorption with recombinant p62 and the use of a novel flow cytometry-based assay for quantitation of nuclear import. The purified activity (NTF2) is an apparent dimer of approximately 14-kD subunits and is present at approximately 10(6) copies per cell. We obtained a cDNA encoding NTF2 and showed that the recombinant protein restores transport activity to p62-pretreated cytosol. Our data suggest that NTF2 acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. NTF2 interacts with at least one additional cytosolic transport activity, indicating that it could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.

subject areas

  • Amino Acid Sequence
  • Biological Transport
  • Caenorhabditis elegans Proteins
  • Carrier Proteins
  • Cloning, Molecular
  • Cytosol
  • HeLa Cells
  • Humans
  • Integrin beta Chains
  • Integrins
  • Membrane Glycoproteins
  • Molecular Sequence Data
  • Nuclear Envelope
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • Nucleocytoplasmic Transport Proteins
  • Protein Binding
  • Recombinant Proteins
  • Sequence Homology, Amino Acid
  • Subcellular Fractions
  • Time Factors
  • ran GTP-Binding Protein
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Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.129.4.925

PubMed ID

  • 7744965
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Additional Document Info

start page

  • 925

end page

  • 937

volume

  • 129

issue

  • 4

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