Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Mutations in a gene encoding the alpha-subunit of a saccharomyces-cerevisiae g-protein indicate a role in mating pheromone signaling

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Jahng, K. Y.
  • Ferguson, J.
  • Reed, Steven

publication date

  • June 1988

journal

  • Molecular and Cellular Biology  Journal

abstract

  • Mutations which allowed conjugation by Saccharomyces cerevisiae cells lacking a mating pheromone receptor gene were selected. One of the genes defined by such mutations was isolated from a yeast genomic library by complementation of a temperature-sensitive mutation and is identical to the gene GPA1 (also known as SCG1), recently shown to be highly homologous to genes encoding the alpha subunits of mammalian G proteins. Physiological analysis of temperature-sensitive gpa1 mutations suggests that the encoded G protein is involved in signaling in response to mating pheromones. Mutational disruption of G-protein activity causes cell-cycle arrest in G1, deposition of mating-specific cell surface agglutinins, and induction of pheromone-specific mRNAs, all of which are responses to pheromone in wild-type cells. In addition, mutants can conjugate without the benefit of mating pheromone or pheromone receptor. A model is presented where the activated G protein has a negative impact on a constitutive signal which normally keeps the pheromone response repressed.

subject areas

  • Agglutinins
  • Conjugation, Genetic
  • Fungal Proteins
  • GTP-Binding Proteins
  • Mutation
  • Phenotype
  • Pheromones
  • Saccharomyces cerevisiae
  • Transcription, Genetic
  • Transduction, Genetic
scroll to property group menus

Identity

PubMed Central ID

  • PMC363449

International Standard Serial Number (ISSN)

  • 0270-7306

PubMed ID

  • 3136318
scroll to property group menus

Additional Document Info

start page

  • 2484

end page

  • 2493

volume

  • 8

issue

  • 6

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support