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Proteomic profiling of the influence of iron availability on Cryptococcus gattii

Academic Article
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Overview

authors

  • Crestani, J.
  • Carvalho, P. C.
  • Han, X. M.
  • Seixas, A.
  • Broetto, L.
  • Fischer, J. D. D.
  • Staats, C. C.
  • Schrank, A.
  • Yates III, John
  • Vainstein, M. H.

publication date

  • January 2012

journal

  • Journal of Proteome Research  Journal

abstract

  • Iron is essential and ubiquitous in living organisms. The competition for this micronutrient between the host and its pathogens has been related to disease establishment. Cryptococcus gattii is an encapsulated yeast that causes cryptococcosis mainly in immunocompetent individuals. In this study, we analyzed the proteomic profile of the C. gattii R265 Vancouver Island isolate under iron-depleted and -repleted conditions by multidimensional protein identification technology (MudPIT) and by 2D-GE. Proteins and key mechanisms affected by alteration of iron levels such as capsule production, cAMP-signaling pathway, response to stress, and metabolic pathways related to mitochondrial function were identified. Our results also show both proteomic methodologies employed to be complementary.

subject areas

  • Biosynthetic Pathways
  • Cryptococcus gattii
  • Electrophoresis, Gel, Two-Dimensional
  • Fungal Proteins
  • Gene Expression
  • Gene Expression Regulation, Fungal
  • Iron
  • Molecular Sequence Annotation
  • Proteome
  • Proteomics
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Research

keywords

  • 2D-GE
  • 2D-LC-MS/MS
  • Cryptococcus gattii R265
  • MudPIT
  • iron
  • proteomics
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Identity

PubMed Central ID

  • PMC3253262

International Standard Serial Number (ISSN)

  • 1535-3893

Digital Object Identifier (DOI)

  • 10.1021/pr2005296

PubMed ID

  • 21970549
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Additional Document Info

start page

  • 189

end page

  • 205

volume

  • 11

issue

  • 1

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