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Alteration of the retinotectal map in xenopus by antibodies to neural cell-adhesion molecules

Academic Article
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Overview

authors

  • Fraser, S. E.
  • Murray, B. A.
  • Chuong, C. M.
  • Edelman, Gerald

publication date

  • 1984

journal

  • Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences  Journal

abstract

  • The neural cell adhesion molecule (N-CAM) mediates neuron-neuron adhesion, is ubiquitous in the nervous system of developing and mature vertebrates, and undergoes major alterations in both amount and distribution during development. Perturbation of homophilic (N-CAM to N-CAM) binding by univalent fragments of specific anti-N-CAM antibodies has previously been found to alter neural tissue patterns in vitro. To show that significant alterations can also occur in vivo, antibodies to Xenopus N-CAM were embedded in agarose microcylinders and implanted in the tecta of juvenile Xenopus laevis frogs that were undergoing regeneration of their retinotectal projections; 1 week later, the effects of implantation on the projection pattern from the optic nerve were determined. Both polyclonal and monoclonal antibodies to N-CAM distorted the retinotectal projection pattern and greatly decreased the precision of the projection; these alterations recovered to near normal after an additional 3 weeks. Similar but smaller effects were obtained when normally developing froglets received tectal implants. In control animals, implants of immunoglobulins from preimmune serum and monoclonal antibodies not directed against N-CAM had little or no effect on the pattern. The results suggest that neuronal adhesion mediated by N-CAM is important in establishing and maintaining the precision and topography of neural patterns.

subject areas

  • Aging
  • Animals
  • Antibodies
  • Antigen-Antibody Complex
  • Antigens, Surface
  • Cell Adhesion Molecules
  • Electric Conductivity
  • Electric Stimulation
  • Neurons
  • Retina
  • Superior Colliculi
  • Xenopus
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.81.13.4222

PubMed ID

  • 6588385
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Additional Document Info

start page

  • 4222

end page

  • 4226

volume

  • 81

issue

  • 13

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