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Molecular characterization of single memory b-cells

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Overview

authors

  • McHeyzer-Williams, Michael G.
  • Nossal, G. J. V.
  • Lalor, P. A.

publication date

  • 1991

journal

  • Nature  Journal

abstract

  • Primary antigenic exposure results in an initial antibody response and the T cell-dependent induction of B-cell memory. Memory B-cell differentiation is characterized by somatic hypermutation in antibody variable region genes (V) and selection of B cells expressing high-affinity variants of this antigen receptor. Despite our current understanding of B-cell memory, the origin of memory B cells and the regulation of their differentiation remain elusive. This is largely due to the difficulties in observing and purifying this minor component of the immunized spleen. Further, molecular characterization of memory B cells requires hybridoma formation which restricts analyses to only those clones capable of fusion and does not allow isolation of cells in a normal physiological state. We have therefore developed a unique system which allows isolation and unambiguous enumeration of IgG1+ memory B cells, based on six-parameter flow cytometry, secretion of antibody in clonal cultures and analysis of clonally expressed V genes using the polymerase chain reaction. Here we report that single IgG1+ antigen-binding B cells from an early secondary immune response proliferate in lipopolysaccharide-driven microcultures and produce antigen-specific IgG1 antibodies. Individual B-cell clones in these cultures express somatically mutated heavy chain V genes, confirming their designation as memory B cells. Although isolated memory B cells undergo extensive proliferation in vitro, V gene sequence analysis of their individual progeny shows that further hypermutation does not occur.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antibody Formation
  • Antigens
  • B-Lymphocytes
  • Base Sequence
  • Cell Differentiation
  • Cells, Cultured
  • Flow Cytometry
  • Immunoglobulin G
  • Immunoglobulin Variable Region
  • Immunologic Memory
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Polymerase Chain Reaction
  • Receptors, Antigen, B-Cell
  • Spleen
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Identity

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/350502a0

PubMed ID

  • 2014051
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Additional Document Info

start page

  • 502

end page

  • 505

volume

  • 350

issue

  • 6318

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