Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Adhesion among neural cells of chick-embryo .1. Immunological assay for molecules involved in cell-cell binding

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Brackenbury, R.
  • Thiery, J. P.
  • Rutishauser, U.
  • Edelman, Gerald

publication date

  • 1977

journal

  • Journal of Biological Chemistry  Journal

abstract

  • An immunologically based method for the quantitative assay of molecules involved in cell adhesion is described. Three observations served as a basis for this assay: (a) cells obtained by trypsinization of retinal tissue aggregated rapidly, provided they had been allowed to recover in culture from the dissociation process; (b) treatment of chick retinal cells with Fab' fragments from rabbit antibodies against these cells prevented their aggregation; and (c) incubation of these antibody fragments with antigens released by retinal cells in culture neutralized their ability to inhibit aggregation. The amount of neutralizing antigen was determined by measuring the rates of cell aggregation in the presence and absence of antibody and antigen using a particle counter. Although adhesion was inhibited by anti-retinal cell antibodies, it was not affected by lectins or anti-carbohydrate antibodies that also were bound to the cell surface. Together, the results suggest that the inhibition involved blockade or inactivation of particular cell surface molecules and that the retinal cell antigens capable of neutralizing the antibodies represented these molecules or their fragments. In the accompanying paper, we describe the use of this assay for the purification from culture supernatants of a cell surface molecule involved in cell to cell adhesion.

subject areas

  • Animals
  • Antibodies
  • Cell Adhesion
  • Chick Embryo
  • Concanavalin A
  • Immunoglobulin Fab Fragments
  • Methods
  • Retina
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 893445
scroll to property group menus

Additional Document Info

start page

  • 6835

end page

  • 6840

volume

  • 252

issue

  • 19

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support