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Genetic ablation of a mouse gene expressed specifically in brain

Academic Article
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Overview

authors

  • Kingsley, D. M.
  • Rinchik, E. M.
  • Russell, L. B.
  • Ottiger, H. P.
  • Sutcliffe, J. Gregor
  • Copeland, N. G.
  • Jenkins, N. A.

publication date

  • February 1990

journal

  • EMBO Journal  Journal

abstract

  • The 1B1075 gene was initially identified from a cDNA clone of a rat brain messenger RNA expressed in particular subsets of CNS neurons and pituitary cells. Although the protein encoded by this gene is of unknown function, its sequence suggests that it may be related to secretogranin proteins, which are found in association with secretory granules in a variety of peptidergic endocrine and neuronal cells. Here we show that the mouse 1B1075 gene is located between the dilute (d) and short ear (se) genes on chromosome 9. Many different deletion mutations have previously been isolated in the genetic region that includes these genes. By producing mice carrying two deletions that overlap at the 1B1075 locus, the gene for this brain-specific message can be completely eliminated from otherwise viable animals. The animals missing the 1B1075 gene provide an important new tool for determining the function of this gene in the brain. In addition, these results provide a new molecular entry point for detailed characterization of other genes in the d-se region.

subject areas

  • Animals
  • Brain
  • Chromosome Deletion
  • Chromosome Mapping
  • Crosses, Genetic
  • DNA
  • DNA Probes
  • Female
  • Genes
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Nerve Tissue Proteins
  • Nucleic Acid Hybridization
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Identity

PubMed Central ID

  • PMC551679

International Standard Serial Number (ISSN)

  • 0261-4189

PubMed ID

  • 2303033
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Additional Document Info

start page

  • 395

end page

  • 399

volume

  • 9

issue

  • 2

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