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The amino acid sequence of the acidic subunit B-chain of crotoxin

Academic Article
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Overview

authors

  • Aird, S. D.
  • Yates III, John
  • Martino, P. A.
  • Shabanowitz, J.
  • Hunt, D. F.
  • Kaiser, II

publication date

  • September 1990

journal

  • Biochimica et Biophysica Acta  Journal

abstract

  • The B-chain of the acidic subunit of crotoxin proved refractory to Edman degradation. When subjected to sequence analysis using tandem mass spectrometry, pyroglutamate was found at the amino-terminal end, even though earlier attempts to de-block with pyroglutamate aminopeptidase were unsuccessful. The B-chain contained 35 amino acids and showed 91% amino acid identity with the corresponding segment from Mojave toxin, a homologous neurotoxin from Crotalus scutulatus scutulatus. The sequence of the last 24 residues of the B-chain is consistent with that previously published (Aird, S.D., Kaiser, I.I., Lewis, R.V. and Kruggel, W.G. (1985) Biochemistry 24, 7054-7058), except at position 20, where Edman degradation gave glycine and mass spectrometry gave glutamic acid.

subject areas

  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid
  • Crotalid Venoms
  • Crotoxin
  • Fourier Analysis
  • Macromolecular Substances
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptide Fragments
  • Phospholipases A
  • Sequence Homology, Nucleic Acid
  • Snakes
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Identity

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0167-4838(90)90079-u

PubMed ID

  • 2400773
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Additional Document Info

start page

  • 217

end page

  • 224

volume

  • 1040

issue

  • 2

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