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Cip1 and Cip2 are novel RNA-recognition-motif proteins that counteract Csx1 function during oxidative stress

Academic Article
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Overview

authors

  • Martin, V.
  • Rodriguez-Gabriel, M. A.
  • McDonald, W. H.
  • Watt, S.
  • Yates III, John
  • Bahler, J.
  • Russell, Paul

publication date

  • March 2006

journal

  • Molecular Biology of the Cell  Journal

abstract

  • Eukaryotic cells reprogram their global patterns of gene expression in response to stress. Recent studies in Schizosaccharomyces pombe showed that the RNA-binding protein Csx1 plays a central role in controlling gene expression during oxidative stress. It does so by stabilizing atf1(+) mRNA, which encodes a subunit of a bZIP transcription factor required for gene expression during oxidative stress. Here, we describe two related proteins, Cip1 and Cip2, that were identified by multidimensional protein identification technology (MudPIT) as proteins that coprecipitate with Csx1. Cip1 and Cip2 are cytoplasmic proteins that have RNA recognition motifs (RRMs). Neither protein is essential for viability, but a cip1Delta cip2Delta strain grows poorly and has altered cellular morphology. Genetic epistasis studies and whole genome expression profiling show that Cip1 and Cip2 exert posttranscriptional control of gene expression in a manner that is counteracted by Csx1. Notably, the sensitivity of csx1Delta cells to oxidative stress and their inability to induce expression of Atf1-dependent genes are partially rescued by cip1Delta and cip2Delta mutations. This study emphasizes the importance of a modulated mRNA stability in the eukaryotic stress response pathways and adds new information to the role of RNA-binding proteins in the oxidative stress response.

subject areas

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Gene Expression Regulation, Fungal
  • Hydrogen Peroxide
  • Molecular Sequence Data
  • Mutation
  • Oxidative Stress
  • Phenotype
  • RNA, Messenger
  • RNA-Binding Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
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Identity

PubMed Central ID

  • PMC1382307

International Standard Serial Number (ISSN)

  • 1059-1524

Digital Object Identifier (DOI)

  • 10.1091/mbc.E05-09-0847

PubMed ID

  • 16407405
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Additional Document Info

start page

  • 1176

end page

  • 1183

volume

  • 17

issue

  • 3

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