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Quantitative analysis of age specific variation in the abundance of human female parotid salivary proteins

Academic Article
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Overview

authors

  • Ambatipudi, K. S.
  • Lu, B. W.
  • Hagen, F. K.
  • Melvin, J. E.
  • Yates III, John

publication date

  • November 2009

journal

  • Journal of Proteome Research  Journal

abstract

  • Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of pooled saliva collected from the parotid glands of healthy female subjects, divided into two age groups 1 and 2 (20-30 and 55-65 years old, respectively). Hydrophobic charge interaction chromatography was used to separate high- from low-abundance proteins prior to characterization of the parotid saliva using multidimensional protein identification technology (MudPIT). Collectively, 532 proteins were identified in the two age groups. Of these proteins, 266 were identified exclusively in one age group, while 266 proteins were common to both groups. The majority of the proteins identified in the two age groups belonged to the defense and immune response category. Of note, several defense related proteins (e.g., lysozyme, lactoferrin and histatin-1) were significantly more abundant in group 2 as determined by G-test. Selected representative mass spectrometric findings were validated by Western blot analysis. Our study reports the first quantitative analysis of differentially regulated proteins in ductal saliva collected from young and older female subjects. This study supports the use of high-throughput proteomics as a robust discovery tool. Such results provide a foundation for future studies to identify specific salivary proteins which may be linked to age-related diseases specific to women.

subject areas

  • Adult
  • Aged
  • Aging
  • Biomarkers
  • Blotting, Western
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Middle Aged
  • Parotid Gland
  • Proteomics
  • Reproducibility of Results
  • Salivary Proteins and Peptides
  • Young Adult
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Research

keywords

  • Hydrophobic Charge Interaction Chromatography
  • Mass Spectrometry
  • Parotid Saliva
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Identity

PubMed Central ID

  • PMC2834885

International Standard Serial Number (ISSN)

  • 1535-3893

Digital Object Identifier (DOI)

  • 10.1021/pr900478h

PubMed ID

  • 19764810
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Additional Document Info

start page

  • 5093

end page

  • 5102

volume

  • 8

issue

  • 11

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