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An optimal Mg(2+) concentration for kinetic folding of the tetrahymena ribozyme

Academic Article
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Overview

authors

  • Rook, M. S.
  • Treiber, D. K.
  • Williamson, James

publication date

  • October 1999

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Divalent metal ions, such as Mg(2+), are generally required for tertiary structure formation in RNA. Although the role of Mg(2+) binding in RNA-folding equilibria has been studied extensively, little is known about the role of Mg(2+) in RNA-folding kinetics. In this paper, we explore the effect of Mg(2+) on the rate-limiting step in the kinetic folding pathway of the Tetrahymena ribozyme. Analysis of these data reveals the presence of a Mg(2+)-stabilized kinetic trap that slows folding at higher Mg(2+) concentrations. Thus, the Tetrahymena ribozyme folds with an optimal rate at 2 mM Mg(2+), just above the concentration required for stable structure formation. These results suggest that thermodynamic and kinetic folding of RNA are cooptimized at a Mg(2+) concentration that is sufficient to stabilize the folded form but low enough to avoid kinetic traps and misfolding.

subject areas

  • Animals
  • Base Sequence
  • Kinetics
  • Magnesium
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Protein Folding
  • RNA, Catalytic
  • Tetrahymena
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.96.22.12471

PubMed ID

  • 10535946
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Additional Document Info

start page

  • 12471

end page

  • 12476

volume

  • 96

issue

  • 22

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