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Template-directed synthesis on the pentanucleotide CpCpGpCpC

Academic Article
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Overview

authors

  • Inoue, T.
  • Joyce, Gerald
  • Grzeskowiak, K.
  • Orgel, L. E.
  • Brown, J. M.
  • Reese, C. B.

publication date

  • 1984

journal

  • Journal of Molecular Biology  Journal

abstract

  • The pentanucleotide CpCpGpCpC facilitates the synthesis of oligomers containing G and C from a mixture of the two activated mononucleotides (guanosine 5'-phosphor)-2-methylimidazolide and (cytidine 5'-phosphor)-2-methylimidazolide. The major pentameric product of the template-directed reaction is all 3' to 5'-linked and has the sequence pGpGpCpGpG, which is complementary to that of the template. It can be obtained in a yield of up to 17%, based on the input of the template. The 3' to 5' isomer of GpG is elongated on the template to give GpGpC, GpGpCpG and GpGpCpGpG, while the 2' to 5' isomer does not initiate the synthesis of detectable amounts of longer oligomers.

subject areas

  • Chromatography, High Pressure Liquid
  • Cytidine Monophosphate
  • Guanosine Monophosphate
  • Oligonucleotides
  • Templates, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/0022-2836(84)90244-4

PubMed ID

  • 6092644
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Additional Document Info

start page

  • 669

end page

  • 676

volume

  • 178

issue

  • 3

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