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Amplification, rearrangement, and elevated expression of c-myc in the human prostatic carcinoma cell line LNCaP

Academic Article
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Overview

authors

  • Nag, A.
  • Smith, Roy

publication date

  • 1989

journal

  • Prostate  Journal

abstract

  • We have investigated the structure and expression of the c-myc proto-oncogene in DNA isolated from the immortal cell line LNCaP. This cell line was derived from a lymph node metastasis of human prostate cancer. Msp I digest of LNCaP DNA when hybridized to a human c-myc probe showed a 1.45 kb band of intensity about two-fold greater than that observed in normal lymphocytes. In addition, the LNCaP cells contain rearranged and amplified c-myc structures which are not present in normal lymphocytes. Quantitation of these bands by scanning densitometry is consistent with an approximately 10-fold amplification of c-myc. To determine whether this amplification was accompanied by increased expression, RNA was isolated from these cells and compared to RNA isolated from a control cell line in which c-myc was not amplified. Northern blot analysis showed that the RNA transcripts from LNCaP cells were approximately 50-fold higher. Although androgens modulate the cell growth of LNCaP, there was no change in the level of c-myc RNA transcripts in serum-free medium in the presence or absence of androgens. Further investigation to determine whether altered structure, amplification, and overexpression of c-myc constitute a common characteristic of metastatic human prostate cancer might prove profitable in understanding this disease.

subject areas

  • DNA, Neoplasm
  • Gene Amplification
  • Gene Expression
  • Gene Rearrangement
  • Humans
  • Immunoblotting
  • Male
  • Mutation
  • Oncogenes
  • Prostatic Neoplasms
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogene Proteins p21(ras)
  • Proto-Oncogenes
  • Testosterone
  • Tumor Cells, Cultured
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Identity

International Standard Serial Number (ISSN)

  • 0270-4137

Digital Object Identifier (DOI)

  • 10.1002/pros.2990150205

PubMed ID

  • 2678039
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Additional Document Info

start page

  • 115

end page

  • 122

volume

  • 15

issue

  • 2

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