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Engineered streptomyces platensis strains that overproduce antibiotics platensimycin and platencin

Academic Article
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Overview

authors

  • Smanski, M. J.
  • Peterson, R. M.
  • Rajski, S. R.
  • Shen, Ben

publication date

  • April 2009

journal

  • Antimicrobial Agents and Chemotherapy  Journal

abstract

  • Platensimycin, which is isolated from Streptomyces platensis MA7327, and platencin, which is isolated from S. platensis MA7339, are two recently discovered natural products that serve as important antibiotic leads. Here we report on the identification of S. platensis MA7327 as a dual producer of both platensimycin and platencin. A PCR-based approach was used to locate and clone the locus involved in platensimycin and platencin production, including ptmR1, which encodes a putative GntR-like transcriptional regulator. Deletion of this gene from the producing organism allowed us to isolate strains that overproduce platensimycin and platencin with yields of 323 +/- 29 mg/liter and 255 +/- 30 mg/liter, respectively. These results illustrate the effectiveness of genetic manipulation for the rational engineering of improvements in titers.

subject areas

  • Adamantane
  • Aminobenzoates
  • Aminophenols
  • Anilides
  • Anti-Bacterial Agents
  • Fermentation
  • Genetic Engineering
  • Hydro-Lyases
  • Multigene Family
  • Polycyclic Compounds
  • Polymerase Chain Reaction
  • Streptomyces
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Identity

PubMed Central ID

  • PMC2663125

International Standard Serial Number (ISSN)

  • 0066-4804

Digital Object Identifier (DOI)

  • 10.1128/aac.01358-08

PubMed ID

  • 19164156
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Additional Document Info

start page

  • 1299

end page

  • 1304

volume

  • 53

issue

  • 4

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