Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Nucleotide determinants for tRNA-dependent amino acid discrimination by a class I tRNA synthetase

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Nordin, Brian, Ph.D. in Biology, Scripps Research 1997 - 2002

authors

  • Farrow, M. A.
  • Nordin, Brian
  • Schimmel, Paul

publication date

  • December 1999

journal

  • Biochemistry  Journal

abstract

  • The high accuracy of the genetic code relies on the ability of tRNA synthetases to discriminate rigorously between closely similar amino acids. While the enzymes can detect differences between closely similar amino acids at an accuracy of about 1 part in 100-200, a finer discrimination requires the presence of the cognate tRNA. The role of the tRNA is to direct the misactivated amino acid to a distinct catalytic site for editing where hydrolysis occurs. Previous work showed that three nucleotides at the corner of the L-shaped tRNA were collectively required. Here we show that each of these nucleotides individually contributes to the efficiency of editing. However, all are dispensable for the chemical step of hydrolysis. Instead, these nucleotides are required for translocation of a misactivated amino acid from the active site to the center for editing.

subject areas

  • Acylation
  • Adenosine Triphosphatases
  • Binding Sites
  • Isoleucine-tRNA Ligase
  • Mutagenesis, Site-Directed
  • Nucleotides
  • RNA Editing
  • RNA, Transfer, Ile
  • RNA, Transfer, Val
  • Valine
  • Valine-tRNA Ligase
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi9920782

PubMed ID

  • 10606524
scroll to property group menus

Additional Document Info

start page

  • 16898

end page

  • 16903

volume

  • 38

issue

  • 51

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support