Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Antibody catalyzed hydrolysis of a quorum sensing signal found in gram-negative bacteria

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Xu, Yang, Ph.D. in Chemistry, Scripps Research 2001 - 2007

authors

  • Marin, S. D.
  • Xu, Yang
  • Meijler, M. M.
  • Janda, Kim

publication date

  • March 2007

journal

  • Bioorganic & Medicinal Chemistry Letters  Journal

abstract

  • N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest k(cat), with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.

subject areas

  • 4-Butyrolactone
  • Antibodies, Bacterial
  • Catalysis
  • Gram-Negative Bacteria
  • Hydrolysis
  • Kinetics
  • Pseudomonas aeruginosa
  • Pyocyanine
  • Quorum Sensing
  • Signal Transduction
scroll to property group menus

Research

keywords

  • catalytic antibodies
  • homoserine lactone hydrolysis
  • inhibitor
  • quorum sensing
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0960-894X

Digital Object Identifier (DOI)

  • 10.1016/j.bmcl.2006.12.118

PubMed ID

  • 17254784
scroll to property group menus

Additional Document Info

start page

  • 1549

end page

  • 1552

volume

  • 17

issue

  • 6

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support