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A cell cycle phosphoproteome of the yeast centrosome

Academic Article
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Overview

authors

  • Keck, Jamie
  • Jones, M. H.
  • Wong, C. C. L.
  • Binkley, J.
  • Chen, D. C.
  • Jaspersen, S. L.
  • Holinger, E. P.
  • Xu, T.
  • Niepel, M.
  • Rout, M. P.
  • Vogel, J.
  • Sidow, A.
  • Yates III, John
  • Winey, M.

publication date

  • June 2011

journal

  • Science  Journal

abstract

  • Centrosomes organize the bipolar mitotic spindle, and centrosomal defects cause chromosome instability. Protein phosphorylation modulates centrosome function, and we provide a comprehensive map of phosphorylation on intact yeast centrosomes (18 proteins). Mass spectrometry was used to identify 297 phosphorylation sites on centrosomes from different cell cycle stages. We observed different modes of phosphoregulation via specific protein kinases, phosphorylation site clustering, and conserved phosphorylated residues. Mutating all eight cyclin-dependent kinase (Cdk)-directed sites within the core component, Spc42, resulted in lethality and reduced centrosomal assembly. Alternatively, mutation of one conserved Cdk site within γ-tubulin (Tub4-S360D) caused mitotic delay and aberrant anaphase spindle elongation. Our work establishes the extent and complexity of this prominent posttranslational modification in centrosome biology and provides specific examples of phosphorylation control in centrosome function.

subject areas

  • Binding Sites
  • CDC2 Protein Kinase
  • Cell Cycle
  • Centrosome
  • Cytoskeletal Proteins
  • Fungal Proteins
  • Fungi
  • G1 Phase
  • Mitosis
  • Mutation
  • Phosphoproteins
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Proteome
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Spindle Apparatus
  • Tubulin
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Identity

PubMed Central ID

  • PMC3825980

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1205193

PubMed ID

  • 21700874
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Additional Document Info

start page

  • 1557

end page

  • 1561

volume

  • 332

issue

  • 6037

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