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P53/58 binds COPI and is required for selective transport through the early secretory pathway

Academic Article
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Overview

authors

  • Tisdale, E. J.
  • Plutner, H.
  • Matteson, J.
  • Balch, William E.

publication date

  • May 1997

journal

  • Journal of Cell Biology  Journal

abstract

  • p53/58 is a transmembrane protein that continuously recycles between the ER and pre-Golgi intermediates composed of vesicular-tubular clusters (VTCs) found in the cell periphery and at the cis face of the Golgi complex. We have generated an antibody that uniquely recognizes the p53/58 cytoplasmic tail. Here we present evidence that this antibody arrests the anterograde transport of vesicular stomatitis virus glycoprotein and leads to the accumulation of p58 in pre-Golgi intermediates. Consistent with a role for the KKXX retrieval motif found at the cytoplasmic carboxyl terminus of p53/58 in retrograde traffic, inhibition of transport through VTCs correlates with the ability of the antibody to block recruitment of COPI coats to the p53/58 cytoplasmic tail and to p53/58-containing membranes. We suggest that p53/58 function may be required for the coupled exchange of COPII for COPI coats during segregation of anterograde and retrograde transported proteins.

subject areas

  • Amino Acid Sequence
  • Animals
  • Biological Transport
  • Coated Vesicles
  • Coatomer Protein
  • Cricetinae
  • Cytoplasm
  • Endoplasmic Reticulum, Rough
  • Exocytosis
  • Fluorescent Antibody Technique, Indirect
  • Golgi Apparatus
  • Mannose-Binding Lectins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Microtubule-Associated Proteins
  • Peptides
  • Protein Binding
  • Rats
  • Viral Envelope Proteins
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Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.137.3.581

PubMed ID

  • 9151666
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Additional Document Info

start page

  • 581

end page

  • 593

volume

  • 137

issue

  • 3

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