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Limited functional diversity in kappa-light-chains - junctional sequences from CD43(+)B220(+) early B-cell progenitors resemble those from peripheral B-cells

Academic Article
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Overview

authors

  • Victor, K. D.
  • Vu, K.
  • Feeney, Ann

publication date

  • April 1994

journal

  • Journal of Immunology  Journal

abstract

  • Among all adult T and B cell Ag receptor chains, only Ig light chains lack N regions. It is thought that this is due to the fact that light chain genes rearrange after heavy chain genes, and that terminal deoxynucleotidyl transferase, the enzyme that adds N regions, is not longer expressed at that stage. However, this concept has been challenged recently by the demonstration that 3 to 10% of B cell precursors (CD43+B220+) appear to rearrange their light chains at approximately the same time as they undergo VH-->DJ rearrangements. To examine N region addition in B cell precursors undergoing early kappa-chain rearrangement, we PCR amplified rearranged V kappa 21 genes from the CD43+B220+ bone marrow cells and compared them to sequences obtained from whole bone marrow and spleen. Unexpectedly, all three populations showed approximately 10% N region containing junctions, most consisting of only one N nucleotide. Thus, even the B cell precursors that rearrange light chains at this early stage of development lack much N region diversity. Twelve percent of the sequences unambiguously contained P regions, which were from 1 to 5 nucleotides in length. All but 2 of the 41 productive rearrangements had the commonly observed CDR3 length of nine amino acids. Many (71%) of the sequences were out of frame. CDR3 length was very restricted in nonproductive rearrangements too, and deletion of nucleotides from V kappa and J kappa gene segments was limited. Thus, even at the level of nonproductive rearrangements, junctional diversity is minimal for kappa-chains.

subject areas

  • Animals
  • Antibody Diversity
  • Antigens, CD
  • Antigens, CD43
  • Antigens, CD45
  • Antigens, Surface
  • B-Lymphocytes
  • Base Sequence
  • Bone Marrow Cells
  • Cell Differentiation
  • Gene Rearrangement, B-Lymphocyte, Light Chain
  • Genes, Immunoglobulin
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Sialoglycoproteins
  • Spleen
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 7511648
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Additional Document Info

start page

  • 3467

end page

  • 3475

volume

  • 152

issue

  • 7

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