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Using the process of reactive immunization to induce catalytic antibodies with complex mechanisms: Aldolases

Academic Article
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Overview

authors

  • Lerner, Richard
  • Barbas III, Carlos

publication date

  • August 1996

journal

  • Acta Chemica Scandinavica  Journal

abstract

  • The process of reactive immunization has been used to induce efficient aldolase catalytic antibodies that use the enamine mechanism of natural enzymes. Reactive immunogens are those that react chemically during induction of the immune response. This same reaction is used later in catalysis. In essence one immunizes with the equivalent of a mechanism-based inhibitor. The difference is that instead of inhibiting a mechanism, a mechanism is induced. This advance allows the experimenter to dictate the exact mechanism by which catalytic antibodies proceed. The hapten used in the present study is a 1,3-diketone that both traps the requisite lysine residue to initiate formation of the enamine and induces a binding pocket that overcomes the entropic barrier of this bimolecular reaction.

subject areas

  • Acetone
  • Aldehydes
  • Antibodies
  • Antibodies, Catalytic
  • Binding Sites
  • Enzyme Inhibitors
  • Fructose-Bisphosphate Aldolase
  • Imines
  • Immunization
  • Ketones
  • Kinetics
  • Lysine
  • Molecular Conformation
  • Molecular Structure
  • Spectrophotometry
  • Substrate Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0904-213X

Digital Object Identifier (DOI)

  • 10.3891/acta.chem.scand.50-0672

PubMed ID

  • 8756355
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Additional Document Info

start page

  • 672

end page

  • 678

volume

  • 50

issue

  • 8

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