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A v(h)11v(kappa)9b cell antigen receptor drives generation of cd5(+) b cells both in vivo and in vitro

Academic Article
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Overview

authors

  • Chumley, M. J.
  • Dal Porto, J. M.
  • Kawaguchi, S.
  • Cambier, J. C.
  • Nemazee, David
  • Hardy, R. R.

publication date

  • May 2000

journal

  • Journal of Immunology  Journal

abstract

  • B lymphocytes can be divided into different subpopulations, some with distinctive activation requirements and probably mediating specialized functions, based on surface phenotype and/or anatomical location, but the origins of most of these populations remain poorly understood. B cells constrained by transgenesis to produce an Ag receptor derived from a conventional (B-2) type cell develop a B-2 phenotype, whereas cells from mice carrying a B-1-derived receptor acquire the B-1 phenotype. In this study transgenic enforced expression of a B cell receptor (mu/kappa) originally isolated from a CD5+ (B-1a) B cell generates B-1 phenotype cells in bone marrow cultures that show a distinctive B-1 function, survival in culture. Despite their autoreactivity, we find no evidence for receptor editing or that the paucity of B-2 cells is the result of tolerance-induced selection. Finally, Ca2+ mobilization studies reveal a difference between transgenic B-1 cells in spleen and peritoneal cavity, with cells in spleen much more responsive to anti-B cell receptor cross-linking. We discuss these results in terms of specificity vs lineage models for generation of distinctive B cell subpopulations.

subject areas

  • Adoptive Transfer
  • Animals
  • Antigens, CD5
  • B-Lymphocyte Subsets
  • Bone Marrow Cells
  • Bone Marrow Transplantation
  • Calcium Signaling
  • Cell Differentiation
  • Cell Survival
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • Immunophenotyping
  • Intracellular Fluid
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Peritoneum
  • RNA Editing
  • Receptors, Antigen, B-Cell
  • Spleen
  • Stem Cell Transplantation
  • Stem Cells
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 10779761
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Additional Document Info

start page

  • 4586

end page

  • 4593

volume

  • 164

issue

  • 9

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