Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Receptor editing and marginal zone b cell development are regulated by the helix-loop-helix protein, e2a

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Quong, M. W.
  • Martensson, A.
  • Langerak, A. W.
  • Rivera, R. R.
  • Nemazee, David
  • Murre, C.

publication date

  • 2004

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Previous studies have indicated that the E2A gene products are required to initiate B lineage development. Here, we demonstrate that E2A(+/-) B cells that express an autoreactive B cell receptor fail to mature due in part to an inability to activate secondary immunoglobulin (Ig) light chain gene rearrangement. Both RAG1/2 gene expression and RS deletion are severely defective in E2A(+/-) mice. Additionally, we demonstrate that E2A(+/-) mice show an increase in the proportion of marginal zone B cells with a concomitant decrease in the proportion of follicular B cells. In contrast, Id3-deficient splenocytes show a decline in the proportion of marginal zone B cells. Based on these observations, we propose that E-protein activity regulates secondary Ig gene rearrangement at the immature B cell stage and contributes to cell fate determination of marginal zone B cells. Additionally, we propose a model in which E-proteins enforce the developmental checkpoint at the immature B cell stage.

subject areas

  • Animals
  • B-Lymphocytes
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • DNA Primers
  • DNA-Binding Proteins
  • Gene Expression
  • Gene Rearrangement, B-Lymphocyte, Light Chain
  • Genes, RAG-1
  • Helix-Loop-Helix Motifs
  • Heterozygote
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • RNA Editing
  • Receptors, Antigen, B-Cell
  • TCF Transcription Factors
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors
scroll to property group menus

Research

keywords

  • E-proteins
  • Id3
  • RAG
  • checkpoint
  • follicular B cells
scroll to property group menus

Identity

PubMed Central ID

  • PMC2211894

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.20031180

PubMed ID

  • 15078898
scroll to property group menus

Additional Document Info

start page

  • 1101

end page

  • 1112

volume

  • 199

issue

  • 8

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support