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Protein expression profiles of C3H 10T1/2 murine fibroblasts and of isogenic cells transformed by the H1047R mutant of phosphoinositide 3-kinase (PI3K)

Academic Article
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Overview

authors

  • Hart, J. R.
  • Liao, L. J.
  • Ueno, L.
  • Yates III, John
  • Vogt, Peter K.

publication date

  • March 2011

journal

  • Cell Cycle  Journal

abstract

  • We have used stable isotope labeling with amino acids in cell culture (SILAC) in conjunction with tandem mass spectrometry to characterize the proteomes of two isogenic cell lines that differ in the expression of a single oncoprotein,p110α of PI3K, carrying the H1047R mutation. 51,510 peptides were identified and assigned to 4,201 proteins. Most notable among the proteins that show increased expression in the oncogenically transformed cells are several involved in the interferon response including Isg15, Ifit1, Igtp and Oas2 (interferon stimulated gene 15, interferon-induced protein with tetratricopeptide repeats 1, interferon gamma-inducible GTP-binding protein, 2'-5'-oligoadenylate synthetase 2). Prominent among the downregulated proteins are several involved in cell adhesion as well as proteins that are affected by the negative feedback from PI3K signaling. The differential expressions documented in this analysis suggest novel links between oncogenic PI3K and several signaling pathways. These links will be explored in future studies.

subject areas

  • Amino Acid Substitution
  • Animals
  • Catalytic Domain
  • Cell Line, Transformed
  • Chromatography, High Pressure Liquid
  • Down-Regulation
  • Fibroblasts
  • Isotope Labeling
  • Mice
  • Mutation
  • Phosphatidylinositol 3-Kinases
  • Proteome
  • Signal Transduction
  • Tandem Mass Spectrometry
  • Transfection
  • Up-Regulation
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Research

keywords

  • PI 3-kinase
  • SILAC
  • mass spectrometry
  • oncogenic transformation
  • stable isotope labeling
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Identity

PubMed Central ID

  • PMC3100876

International Standard Serial Number (ISSN)

  • 1538-4101

Digital Object Identifier (DOI)

  • 10.4161/cc.10.6.15077

PubMed ID

  • 21350335
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Additional Document Info

start page

  • 971

end page

  • 976

volume

  • 10

issue

  • 6

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