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Selection and application of peptide-binding peptides

Academic Article
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Overview

authors

  • Zhang, Z. W.
  • Zhu, W. G.
  • Kodadek, Thomas

publication date

  • January 2000

journal

  • Nature Biotechnology  Journal

abstract

  • Peptide-binding ligands would be useful for directing reagents to particular epitopes in a protein, the detection of peptide hormones, and many other applications. Here we show that peptides of modest size isolated from a library using a simple genetic assay can act as specific receptors for other peptides. The equilibrium dissociation constants of these peptide-peptide complexes are higher than those of typical monoclonal antibody-epitope complexes. Nonetheless, as shown here, these peptide-binding peptides can be used to detect or purify proteins containing the partner peptide.

subject areas

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Blotting, Western
  • Chickens
  • Chromatography, Affinity
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Dimerization
  • Epitopes
  • Escherichia coli
  • Genes, Reporter
  • Humans
  • Insulin-Like Growth Factor I
  • Interleukin-1
  • Lac Operon
  • Ligands
  • Molecular Sequence Data
  • Operator Regions, Genetic
  • Peptide Library
  • Peptides
  • Protein Binding
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Two-Hybrid System Techniques
  • Viral Proteins
  • Viral Regulatory and Accessory Proteins
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Research

keywords

  • epitope binding
  • lambda repressor
  • peptides
  • two-hybrid assay
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Identity

International Standard Serial Number (ISSN)

  • 1087-0156

PubMed ID

  • 10625395
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Additional Document Info

start page

  • 71

end page

  • 74

volume

  • 18

issue

  • 1

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