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Messenger-rna coding for oxytocin is present in axons of the hypothalamoneurohypophyseal tract

Academic Article
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Overview

authors

  • Jirikowski, G. F.
  • Sanna, Pietro
  • Bloom, Floyd

publication date

  • October 1990

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Neuronal mRNA is thought to be restricted to perikaryal and dendritic compartments containing rough endoplasmic reticulum. We have used both in situ hybridization and DNA polymerase chain reaction methods to determine the precise intracellular distribution of oxytocin mRNA. Using light- and electron-microscopic detection of in situ hybridization with 5'-bromo-2'-deoxyuridine-labeled oligonucleotide probes, we found oxytocin mRNA in axons and Herring bodies in the lateral and ventral hypothalamus, the median eminence, and the posterior lobe of the pituitary in postpartum lactating rats. Southern blot analysis of the amplification products confirmed the presence of oxytocin mRNA in all three tissue samples. The present findings indicate that oxytocin mRNA can be transported axonally. Such transport could reflect an adventitious compartmentalization or a functional storage in Herring bodies for subsequent secretion.

subject areas

  • Animals
  • Axons
  • Base Sequence
  • Exons
  • Female
  • Hypothalamo-Hypophyseal System
  • Lactation
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Oxytocin
  • Polymerase Chain Reaction
  • Pregnancy
  • RNA, Messenger
  • Rats
  • Rats, Inbred Strains
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.87.19.7400

PubMed ID

  • 2268384
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Additional Document Info

start page

  • 7400

end page

  • 7404

volume

  • 87

issue

  • 19

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